The museum is located in the building of the former Magdeburg Barracks, which served as the headquarters of the so-called Jewish Self-Government during the ghetto era. Today, this building houses extensive exhibitions presenting to the public the unique artistic, musical, literary and theatrical works of the ghetto inhabitants.

During World War II, this building was a home for Jewish boys (10-15 years old). Nowadays there is a museum with an exhibition about the history of the ghetto and the persecution of Jews in the Protectorate of Bohemia and Moravia. Part of the exhibition is dedicated to the youngest victims of the ghetto.