We operate a literary museum. We provide protection of museum collections. We acquire, permanently preserve, process and make accessible collections of documents on the development of literature in the Czech lands. We organize and art exhibitions and programs for the public. We offer rentals of space in the Small Villa.

We operate a lapidarium with a permanent exhibition Monuments of stone masonry in Bohemia from the 11th to the 19th century.

We offer displays on transportation, architecture, construction and design, astronomy and printing. We also have a photography studio, a model hall with a Mercury game room, the Chemistry Around Us exhibition and Measuring Time.

We offer exhibitions, lectures and other events at the National Museum. There is a cloakroom, a café, a children's corner and a shop with publications. The building is wheelchair accessible. We provide access to the exhibition with a pram.

We operate a museum in the premises of a fallout shelter. The exhibition focuses on border and customs guards, public safety, wiretapping and radio professional military equipment, where visitors can try out, for example, Morse code.

We operate a museum focusing on education and education issues. In addition to the permanent exhibition, we also organise short-term exhibitions. We are a research centre focused on the history of Czech education, pedagogy, teaching and education in relation to the life, work and legacy of J. A. Comenius.

Operation of the Museum and Hall of Fame of Czech Hockey. Join us in the world of ice passion, where the past meets the present and the future. Every object, every jersey, every trophy has its own story and together they create a mosaic of exciting moments.

The main mission of the centre is to promote modern Prague interwar and post-war architecture, residential culture, applied arts and lifestyle, represented in different ways in the two buildings, in close connection with two important villas - Müller and Rothmayer.

The museum houses more than 22,000 items. The museum also includes a collection of synthetic crystals and a smaller collection of tektites, comprising 272 items. The museum also houses a student collection used to demonstrate minerals in mineralogy classes.

The museum was founded in 1891 at the instigation of the organisers of the Jubilee Exhibition. The individual branches of the National Museum of Agriculture are aimed at covering agriculture and its relevant fields in their scientific research, acquisition and presentation activities in a comprehensive manner.